7BAH
Structure of RIG-I CTD bound to OH-RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 65 |
| Unit cell lengths | 81.920, 81.920, 111.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.920 - 1.890 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ncu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.590 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.920 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmeas | 0.117 | 0.810 |
| Number of reflections | 33784 | 0 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.4 | |
| Redundancy | 10.9 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 283.15 | 0.1 M Tris-HCl pH 8.0 0.2 M NaCl 18% PEG 3350 |
