7B9P
Structure of Ribonucleotide reductase from Rhodobacter sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 140.748, 140.748, 364.277 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.860 - 2.646 |
R-factor | 0.2312 |
Rwork | 0.230 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ensemble |
Data reduction software | XDS |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.860 | 2.740 |
High resolution limit [Å] | 2.646 | 2.650 |
Rmerge | 0.169 | 7.684 |
Rmeas | 0.174 | 7.886 |
Rpim | 0.040 | 1.756 |
Number of reflections | 62949 | 6078 |
<I/σ(I)> | 13.9 | 0.3 |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 19.29 | 19.7 |
CC(1/2) | 0.999 | 0.148 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | Precipitant: 30% PEG 1500, 20% Glycerol Protein solution: 45 mg/mL Protein + 100 uM dATP Hanging drop experiment: 1.0 uL Protein solution + 1.0 uL Precipitant over 500 uL precipitant |