7B9C

Structure of a minimal SF3B core in complex with spliceostatin A (form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	108.573, 111.066, 248.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.750 - 2.400
R-factor	0.2179
Rwork	0.216
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ife 6en4
RMSD bond length	0.003
RMSD bond angle	0.573
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.750	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.093	4.123
Rmeas	0.097	4.286
Rpim	0.026	1.162
Number of reflections	133845	6530
<I/σ(I)>	18.5	0.7
Completeness [%]	100.0	99.5
Redundancy	13.6	13.3
CC(1/2)	1.000	0.329

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M Bis-Tris Propane pH 7.1-7.45, 0.2 M Potassium thiocyanate (KSCN), 11-12% (w/v) PEG-3350, 10-15% (v/v) Glycerol