7B96

NHL domain of human TRIM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.872900
Spacegroup name	P 21 21 21
Unit cell lengths	44.180, 83.200, 132.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.700 - 1.800
R-factor	0.1782
Rwork	0.177
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qf7
RMSD bond length	0.012
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.700	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.138	1.094
Rmeas	0.155	1.240
Rpim	0.070	0.571
Number of reflections	46050	4531
<I/σ(I)>	6.72	1.24
Completeness [%]	99.5
Redundancy	4.5
CC(1/2)	0.990	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10mg/mL protein in 20mM Tris pH 7.5, 150mM NaCl, 20% (v/v) glycerol and 1mM DTT was added at a 1:1 ratio to the reservoir buffer: 0.2M potassium chloride and 24% (w/v) polyethylene glycol 3350 pH 7.5