7B74

Chimeric Streptavidin With A Dimerization Domain For Artificial Transfer Hydrogenation

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000029
Spacegroup name	P 1 21 1
Unit cell lengths	57.285, 57.307, 88.035
Unit cell angles	90.00, 94.62, 90.00

Refinement procedure

Resolution	47.980 - 1.850
Rwork	0.191
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bc3
RMSD bond length	0.011
RMSD bond angle	2.455
Data reduction software	Aimless (0.7.4)
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.980	47.980	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.159	0.042	1.270
Rmeas	0.188	0.049	1.494
Rpim	0.100	0.026	0.782
Number of reflections	48147	447	2897
<I/σ(I)>	9.2
Completeness [%]	98.9
Redundancy	6.8	6.2	7
CC(1/2)	0.996	0.998	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 %w/v PEG 3350 (Polymer) 0.2 M KF (Salt)