7B6C
BK Polyomavirus VP1 pentamer fusion with long C-terminal extended arm
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.297, 97.205, 146.166 |
| Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
| Resolution | 90.000 - 2.484 |
| Rwork | 0.205 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mj0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.574 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rpim | 0.111 | |
| Number of reflections | 113999 | 11185 |
| <I/σ(I)> | 6.29 | |
| Completeness [%] | 99.0 | 98.22 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.984 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 33 % EDO_P8K,100 mM Morpheus buffer 1 (pH 6.5), 50 mM Morpheus NPS |
