7B5T
S. agalactiae BusR transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 4 |
| Unit cell lengths | 114.180, 114.180, 240.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.800 |
| R-factor | 0.2579 |
| Rwork | 0.257 |
| R-free | 0.26890 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 74011 | 3694 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.9 | |
| CC(1/2) | 1.000 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 150 mM Mg-acetate, 6% D+ Trehalose |
