7B2N
Crystal structure of Chlamydomonas reinhardtii chloroplastic Fructose bisphosphate aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-01-28 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.983995275547 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.493, 251.068, 126.411 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 46.670 - 2.360 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PHYRE2 MODEL |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.200 | 1.495 |
| Number of reflections | 138497 | 13155 |
| <I/σ(I)> | 13.34 | |
| Completeness [%] | 99.4 | 94.4 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.5 M Ammonium sulfate, 6% (v/v) isopropanol |
