7B27

RBD domain SARS-CoV2 in complex with neutralizing nanobody NM1230

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999998
Spacegroup name	P 43 21 2
Unit cell lengths	63.290, 63.290, 411.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.002 - 2.902
Rwork	0.266
R-free	0.30510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xc4 6z1z
RMSD bond length	0.002
RMSD bond angle	1.217
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232 2018/13/08)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.500	2.980
High resolution limit [Å]	2.900	2.900
Rmeas	0.207	1.860
Number of reflections	18493	1340
<I/σ(I)>	7.8	1.15
Completeness [%]	93.5	95.7
Redundancy	6.06
CC(1/2)	0.998	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	293	200 mM MgCl2, 20% (w/v) PEG 3350, pH 5.9