7B1W

Crystal structure of plastidial ribulose epimerase RPE1 from the model alga Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	77.293, 297.322, 77.747
Unit cell angles	90.00, 116.30, 90.00

Refinement procedure

Resolution	49.289 - 1.935
R-factor	0.1846
Rwork	0.184
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model
RMSD bond length	0.008
RMSD bond angle	0.877
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	2.005
High resolution limit [Å]	1.935	1.935
Number of reflections	229414	21621
<I/σ(I)>	9.79
Completeness [%]	98.7
Redundancy	7.2
CC(1/2)	0.997	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Calcium acetate 160 mmol/L Sodium cacodylate pH 6.5 80 mmol/L Glycerol 20 % Polyethylene glycol 8000 14.4 %