7B1V
DtxR-like iron-dependent regulator IdeR complexed with cobalt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-26 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.888, 45.564, 66.275 |
| Unit cell angles | 109.12, 93.20, 113.98 |
Refinement procedure
| Resolution | 61.100 - 2.040 |
| R-factor | 0.2335 |
| Rwork | 0.232 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u8r |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.100 | 2.220 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.058 | 0.579 |
| Rmeas | 0.069 | 0.748 |
| Rpim | 0.037 | 0.465 |
| Number of reflections | 17216 | 862 |
| <I/σ(I)> | 10.3 | 1.5 |
| Completeness [%] | 83.5 | 54.2 |
| Redundancy | 3.3 | 2.4 |
| CC(1/2) | 0.997 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 25%(w/v) PEG 1500 |
