7B0R
In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus with H85R mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.472, 44.871, 69.543 |
| Unit cell angles | 90.00, 121.06, 90.00 |
Refinement procedure
| Resolution | 40.800 - 2.200 |
| Rwork | 0.230 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7b0o |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.150 | 1.420 |
| Number of reflections | 14531 | 1674 |
| <I/σ(I)> | 10.46 | 1.23 |
| Completeness [%] | 92.6 | |
| Redundancy | 6.48 | |
| CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM MES/NaOH, pH 6.0, 125-200 mM M lithium citrate tribasic tetrahydrate, and 36-44 %(v/v) PEG 400 |
