7B0O
In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.724, 44.700, 116.865 |
| Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
| Resolution | 43.320 - 2.330 |
| R-factor | 0.2472 |
| Rwork | 0.245 |
| R-free | 0.28730 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.760 | 2.450 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmeas | 0.290 | 1.450 |
| Number of reflections | 24031 | 1202 |
| <I/σ(I)> | 3.4 | 1.4 |
| Completeness [%] | 91.5 | 56.6 |
| Redundancy | 6.2 | 6.9 |
| CC(1/2) | 0.960 | 0.180 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 293 | 100 mM sodium citrate/HCl, pH 5.6, 40 %(v/v) polyethylene glycol (PEG) 400, 100 mM ammonium sulfate, and 200 mM sodium formate |
| 2 |
