7AZW

Crystal structure of the MIZ1-BTB-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03320
Spacegroup name	I 1 2 1
Unit cell lengths	48.788, 34.518, 172.564
Unit cell angles	90.00, 96.18, 90.00

Refinement procedure

Resolution	35.170 - 2.100
R-factor	0.2411
Rwork	0.239
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m52
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.130	1.079
Number of reflections	17046	1417
<I/σ(I)>	7.4
Completeness [%]	99.8
Redundancy	4.5
CC(1/2)	0.996	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 4000, sodium acetate