7AZT

X-ray crystallographic structure of (6-4)photolyase from Drosophila melanogaster at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	298
Detector technology	CCD
Collection date	2019-05-19
Detector	MPCCD
Wavelength(s)	1.7712
Spacegroup name	P 21 21 21
Unit cell lengths	61.630, 91.640, 115.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.610 - 2.270
R-factor	0.1742
Rwork	0.172
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ayv
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.610	2.351
High resolution limit [Å]	2.270	2.270
Number of reflections	33953	1352
<I/σ(I)>	4.93
Completeness [%]	100.0
Redundancy	106
CC(1/2)	0.970	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	277.15	0.2 M lithium sulftate, 0.1 M bis-tris (pH=6.5), 25% PEG3350, 4% polypropyle glycol P400