7AZE
DNA polymerase sliding clamp from Escherichia coli with peptide 18 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.500, 64.810, 71.100 |
| Unit cell angles | 106.71, 100.07, 116.95 |
Refinement procedure
| Resolution | 52.870 - 1.820 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fvl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.860 | 1.990 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rpim | 0.091 | 1.154 |
| Number of reflections | 60806 | 3041 |
| <I/σ(I)> | 8.5 | 1.3 |
| Completeness [%] | 90.3 | |
| Redundancy | 6 | |
| CC(1/2) | 0.993 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 8% v/v Tacsimate pH 6.0, PEG 3350 20% (w/v) |
