7AZD

DNA polymerase sliding clamp from Escherichia coli with peptide 20 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	69.677, 81.757, 141.926
Unit cell angles	90.00, 97.49, 90.00

Refinement procedure

Resolution	41.390 - 2.190
R-factor	0.191
Rwork	0.189
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fvl
RMSD bond length	0.011
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.690	2.440
High resolution limit [Å]	2.190	2.190
Rpim	0.044	0.524
Number of reflections	58310	2915
<I/σ(I)>	13.1	1.5
Completeness [%]	94.0
Redundancy	13.7
CC(1/2)	0.990	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1M Sodium malonate pH5.0, PEG 3350 12% (w/v)