7AZ5
DNA polymerase sliding clamp from Escherichia coli with peptide 47 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 68.886, 80.166, 83.371 |
Unit cell angles | 62.79, 71.29, 81.66 |
Refinement procedure
Resolution | 27.350 - 1.870 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fvl |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.240 | 2.030 |
High resolution limit [Å] | 1.870 | 1.870 |
Rpim | 0.135 | 0.526 |
Number of reflections | 90577 | 4529 |
<I/σ(I)> | 11.1 | 1.1 |
Completeness [%] | 93.5 | |
Redundancy | 7 | |
CC(1/2) | 0.990 | 0.560 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.2 M Sodium formate pH 7.2, PEG 3350 20% (w/v) |