7AXU

Structure of WDR5:CS-VIP8 cocrystal after illumination in situ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	47.387, 47.172, 133.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.700 - 1.680
R-factor	0.16064
Rwork	0.159
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iam
RMSD bond length	0.016
RMSD bond angle	1.771
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.028	0.198
Rmeas	0.040	0.280
Rpim	0.028	0.198
Number of reflections	34930	6864
<I/σ(I)>	15.39	3.5
Completeness [%]	99.8	99.8
Redundancy	2	2
CC(1/2)	0.998	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	10% (w/v) PEG20000, 20% (v/v) PEG550 MME, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate