7AXS

Structural characterisation of WDR5:CS-VIP8 interaction in cis state 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	47.022, 47.163, 133.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.468 - 1.880
R-factor	0.2013
Rwork	0.200
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iam
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.510	1.948
High resolution limit [Å]	1.880	1.880
Rmerge	0.029	0.224
Rmeas	0.040	0.317
Rpim	0.029	0.224
Number of reflections	22302	3521
<I/σ(I)>	13.12	2.78
Completeness [%]	89.5	77.66
Redundancy	1.9	1.9
CC(1/2)	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	10% (w/v) PEG20000, 20% (v/v) PEG550 MME, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate