7AXQ

Structure of the cryo-trapped WDR5:CS-VIP8 cocrystal after illumination at 405 nm and 180 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	47.000, 47.250, 133.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.547 - 1.562
R-factor	0.1691
Rwork	0.167
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iam
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.590	1.618
High resolution limit [Å]	1.560	1.562
Rmerge	0.090	1.096
Number of reflections	42057	3264
<I/σ(I)>	10.78	1.34
Completeness [%]	97.6
Redundancy	6.4
CC(1/2)	0.999	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	10% (w/v) PEG20000, 20% (v/v) PEG550 MME, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate