7AVT
Crystal structure of SOS1 in complex with compound 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.187, 39.196, 175.938 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 39.010 - 1.880 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7avi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.830 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.980 | 2.103 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.077 | 0.583 |
| Number of reflections | 62069 | 1242 |
| <I/σ(I)> | 10.6 | 1.7 |
| Completeness [%] | 65.7 | 11.7 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 6-11 % PEG 8000, 2 mM DTT, 60 mM Tris |
