7AUG
Crystal structure of rsGCamP1.3 in the ON state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.852, 121.852, 96.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.440 - 2.040 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ya9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.780 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 46902 | 7135 |
| <I/σ(I)> | 9.7 | 1.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.997 | 0.484 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M Na Formate, 0.1 M BTP pH 8.5, 22% PEG 3350 |
