7AQZ
Co-Crystal Structure of Variant Surface Glycoprotein VSG2 in complex with Nanobody VSG2(NB14)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-19 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.542, 95.950, 124.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.237 - 1.300 |
R-factor | 0.1522 |
Rwork | 0.151 |
R-free | 0.17230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vsg 5lhr(chainB) |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.240 | 1.346 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 266024 | 23312 |
<I/σ(I)> | 17.72 | |
Completeness [%] | 98.3 | |
Redundancy | 12.3 | |
CC(1/2) | 1.000 | 0.552 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.1 M citrate, 23 % PEG 1500 |