7AQH
Cell wall binding domain of the Staphylococcal phage 2638A endolysin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 62.130, 62.510, 66.171 |
Unit cell angles | 111.01, 108.39, 90.18 |
Refinement procedure
Resolution | 44.110 - 2.490 |
R-factor | 0.2517 |
Rwork | 0.250 |
R-free | 0.28590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5leo |
RMSD bond length | 0.002 |
RMSD bond angle | 0.469 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.110 | 2.570 |
High resolution limit [Å] | 2.488 | 2.488 |
Rmerge | 0.087 | 0.678 |
Rmeas | 0.117 | 0.907 |
Rpim | 0.077 | 0.598 |
Number of reflections | 51577 | 2454 |
<I/σ(I)> | 4.31 | 0.68 |
Completeness [%] | 90.9 | 79.34 |
Redundancy | 2.06 | |
CC(1/2) | 0.992 | 0.486 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | Protein buffer: 20 mM Tris, 150 mM NaCl, pH 7.4 Crystallization buffer: 1% (W/V) tryptone, 0.05 M HEPES, pH 7.0, 12% (w/v) PEG 3350 |