7APZ
CLIP peptide bound to chicken MHC class II molecule (BL-2) from B2 haplotype with a decamer mode of binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-12-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 82.803, 82.803, 263.071 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 69.280 - 1.970 |
R-factor | 0.2122 |
Rwork | 0.210 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6t3y |
RMSD bond length | 0.009 |
RMSD bond angle | 1.644 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 71.710 | 71.710 | 2.080 |
High resolution limit [Å] | 1.970 | 6.240 | 1.970 |
Rmerge | 0.527 | 0.099 | 5.857 |
Rmeas | 0.535 | 0.101 | 5.941 |
Rpim | 0.089 | 0.019 | 0.991 |
Total number of observations | 1367042 | 38845 | 196438 |
Number of reflections | 38197 | 1452 | 5497 |
<I/σ(I)> | 10.8 | 34.1 | 1.4 |
Completeness [%] | 98.8 | 99.9 | 100 |
Redundancy | 35.8 | 26.8 | 35.7 |
CC(1/2) | 0.996 | 0.994 | 0.417 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M Zinc Acetate (Salt) 0.1 M Imidazole pH 6.5 (Buffer) 10 %w/v PEG 8000 (Precipitant) |