7APJ

Structure of autoinhibited Akt1 reveals mechanism of PIP3-mediated activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-05
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	70.008, 72.197, 120.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.550 - 2.050
R-factor	0.2129
Rwork	0.211
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1unp 4ekk 3ezj
RMSD bond length	0.002
RMSD bond angle	0.507
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.130
High resolution limit [Å]	2.050	2.050
Number of reflections	38951	3750
<I/σ(I)>	9.7
Completeness [%]	99.8	99.1
Redundancy	8.1	7.9
CC(1/2)	0.998	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	200 mM malonate, pH 5.0, 16% PEG 3350