7AP7

Structure of the W64R amyloidogenic variant of human lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980119943619
Spacegroup name	P 21 21 21
Unit cell lengths	31.773, 56.655, 60.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.298 - 1.150
R-factor	0.1623
Rwork	0.161
R-free	0.19270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jsf
RMSD bond length	0.022
RMSD bond angle	1.982
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.4.0)
Refinement software	PHENIX (1.10)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.384	41.384	1.220
High resolution limit [Å]	1.150	3.430	1.150
Rmerge	0.084	0.053	0.769
Number of reflections	39372	1640	6079
<I/σ(I)>	10.04	30.07	1.75
Completeness [%]	98.7	99.8	95.1
Redundancy	6.35
CC(1/2)	0.998	0.996	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.25M (NH4)2SO4, 0.1M cacodylate pH 6.5, 10 mM Tris pH 7, 14.4 TP buffer