7ANY
Structure of the Laspartomycin C Friulimicin-like mutant in complex with Geranyl phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-18 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.132, 68.321, 40.132 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 34.755 - 1.135 |
| R-factor | 0.1613 |
| Rwork | 0.160 |
| R-free | 0.19210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o0z |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.186 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.755 | 1.271 |
| High resolution limit [Å] | 1.135 | 1.135 |
| Rmerge | 0.087 | 0.373 |
| Rmeas | 0.103 | 0.481 |
| Rpim | 0.055 | 0.300 |
| Number of reflections | 36022 | 1799 |
| <I/σ(I)> | 6.2 | 2.8 |
| Completeness [%] | 85.3 | 31.6 |
| Redundancy | 3.3 | 1.9 |
| CC(1/2) | 0.995 | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M cadmium chloride, 40% MPD |
