7AMX

The crystal structure of gene product PA4063 from Pseudomonas aeruginosa in complex with zinc

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-07
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.96770
Spacegroup name	P 41 21 2
Unit cell lengths	62.467, 62.467, 102.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.210 - 2.850
Rwork	0.185
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ahw
RMSD bond length	0.017
RMSD bond angle	1.686
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.470	62.470	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge	0.203	0.136	3.102
Rmeas	0.212	0.143	3.249
Rpim	0.062	0.043	0.962
Number of reflections	5107	204	718
<I/σ(I)>	12.5
Completeness [%]	99.6
Redundancy	20.5	16.1	20.7
CC(1/2)	0.989	0.983	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	ammonium sulfate 2.3M, NaCl 2.1M