7AMU

Crystal structure of rsEGFP2 T204A in its fluorescent on-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	51.269, 61.809, 70.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.570 - 1.640
R-factor	0.1715
Rwork	0.169
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o89
RMSD bond length	0.010
RMSD bond angle	1.741
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	REFMAC (5.8.0258)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.580	1.670
High resolution limit [Å]	1.640	1.640
Rmeas	0.047	1.547
Number of reflections	28151	1356
<I/σ(I)>	14.3	1.2
Completeness [%]	99.4	98.7
Redundancy	4.3	4.4
CC(1/2)	0.999	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	11	293	Ammonium sulphate