7AM0
GqqA- a novel type of quorum quenching acylases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.872, 65.760, 102.660 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 49.350 - 2.500 |
| R-factor | 0.23434 |
| Rwork | 0.232 |
| R-free | 0.27735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7alz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.350 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.040 | 0.520 |
| Number of reflections | 34461 | 3364 |
| <I/σ(I)> | 29.35 | |
| Completeness [%] | 99.4 | 98.53 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 1.000 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.8 M Sodium succinate |
