7ALT

Structure of Drosophila Serrate C2-DSL-EGF1-EGF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	70.329, 49.402, 93.123
Unit cell angles	90.00, 110.25, 90.00

Refinement procedure

Resolution	45.610 - 2.030
R-factor	0.222
Rwork	0.220
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mw7
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (dev_3965)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.610	2.103
High resolution limit [Å]	2.030	2.030
Number of reflections	38729	3814
<I/σ(I)>	11.1
Completeness [%]	98.9
Redundancy	3.3
CC(1/2)	0.998	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1M imidazole malate pH 7, 25% PEG4K