7ALM

Crystal structure of human GDAP1 at 2.8 Angstrom resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	72.808, 115.878, 116.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.360 - 2.800
R-factor	0.2436
Rwork	0.243
R-free	0.26150
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.462
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	CRANK2
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.360	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.040	0.362
Rmeas	0.056	0.512
Rpim	0.040	0.362
Number of reflections	24883	3571
<I/σ(I)>	11.55	2.04
Completeness [%]	99.6	100
Redundancy	2	2
CC(1/2)	0.997	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Magnesium formate, 20 % PEG3350