7ALL
A single sulfatase is required for metabolism of colonic mucin O-glycans and intestinal colonization by a symbiotic human gut bacterium (BT4683-S1_4)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.136, 87.799, 101.833 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.585 - 1.630 |
| Rwork | 0.174 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hdh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.530 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.585 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 62376 | 3024 |
| <I/σ(I)> | 7.8 | 1.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.999 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20 % PEG 3350, 0.2 M sodium iodide and BTP pH 8.5 25mg/ml |
