7AL9
human GTP cyclohydrolase I feedback regulatory protein (GFRP) in complex with phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-17 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.939, 98.123, 67.455 |
| Unit cell angles | 90.00, 102.53, 90.00 |
Refinement procedure
| Resolution | 54.680 - 1.745 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7acc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.680 | 1.980 |
| High resolution limit [Å] | 1.745 | 1.745 |
| Number of reflections | 56173 | 16863 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 82.7 | 76.3 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.999 | 0.344 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 0.1 M NaCit pH 5.0; 20% w/v PEG 8000 (Proplex E11) |
