7AKW
Crystal structure of the viral rhodopsins chimera O1O2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9780 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 44.405, 103.396, 111.381 |
| Unit cell angles | 90.00, 90.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.960 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sqg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.053 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.330 | 41.330 | 2.000 |
| High resolution limit [Å] | 1.960 | 8.970 | 1.960 |
| Rmerge | 0.051 | 0.021 | 1.191 |
| Rmeas | 0.061 | 0.025 | 1.407 |
| Rpim | 0.032 | 0.013 | 0.742 |
| Total number of observations | 126043 | 1293 | 8393 |
| Number of reflections | 36098 | 382 | 2448 |
| <I/σ(I)> | 12.2 | 51.3 | 0.9 |
| Completeness [%] | 99.5 | 97.8 | 95.4 |
| Redundancy | 3.5 | 3.4 | 3.4 |
| CC(1/2) | 0.999 | 0.999 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.6 | 293 | 1.8 M Na2HPO4/KH2PO4 (pH 4.6) |
