7AIA

Complex of human GDAP1 with hexadecanedioic acid

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	73.045, 114.914, 116.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.629 - 2.200
Rwork	0.202
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GDAP1
RMSD bond length	0.014
RMSD bond angle	1.790
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.630	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	50525	4965
<I/σ(I)>	12.72
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	0.998	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Lithium sulfate, 0.1 M bis-Tris pH 5.5, 25% w/v PEG 3350