7AI9

Structure of Ribonucleotide reductase R2 from Escherichia coli collected by rotation serial crystallography on a COC membrane at a synchrotron source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	90.510, 90.510, 207.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.260 - 2.000
R-factor	0.1711
Rwork	0.170
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mxr
RMSD bond length	0.009
RMSD bond angle	0.983
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.260	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.197	11.050
Number of reflections	64377	4762
<I/σ(I)>	10.05	0.48
Completeness [%]	100.0	99.9
Redundancy	26.38	23.1
CC(1/2)	0.999	0.183

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	34-37 % PAA 2100, 100 mM HEPES 7.0, 250-450 mM NaCl, 200 mM ammonium sulfate [and] 26% PAA 2100, 100 mM HEPES 7.0, 150 NaCl, 100 Malonate