7AI8

Structure of Ribonucleotide reductase R2 from Escherichia coli collected by still serial crystallography on a COC membrane at a synchrotron source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	90.350, 90.350, 208.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.970 - 2.100
R-factor	0.1914
Rwork	0.190
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mxr
RMSD bond length	0.007
RMSD bond angle	0.831
Data reduction software	CrystFEL (0.7 / 0.8)
Data scaling software	CrystFEL (0.7 / 0.8)
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.200	2.130
High resolution limit [Å]	2.080	2.100
Number of reflections	31083	1474
<I/σ(I)>	10.74	1.74
Completeness [%]	99.7	96.66
Redundancy	201.59	15.2
CC(1/2)	0.997	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	34-37 % PAA 2100, 100 mM HEPES 7.0, 250-450 mM NaCl, 200 mM ammonium sulfate [and] 26% PAA 2100, 100 mM HEPES 7.0, 150 NaCl, 100 Malonate