7AI4
Crystal structure of the KLC1-TPR domain truncated from its nonTPR region ([A1-B6]-Delta-nonTPR fragment)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978570 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.220, 55.820, 103.570 |
Unit cell angles | 90.00, 108.51, 90.00 |
Refinement procedure
Resolution | 49.110 - 2.795 |
R-factor | 0.2187 |
Rwork | 0.217 |
R-free | 0.25150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5oj8 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.110 | 2.960 |
High resolution limit [Å] | 2.795 | 2.795 |
Number of reflections | 16666 | 2608 |
<I/σ(I)> | 10.17 | 0.92 |
Completeness [%] | 99.0 | 96.7 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.998 | 0.538 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 1.1 M sodium formate and 0.1 M Hepes pH 7.9 |