7AHY
Crystal structure of Western clawed frog MDM2 RING domain homodimer
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.559, 23.760, 55.744 |
| Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
| Resolution | 41.745 - 2.532 |
| Rwork | 0.217 |
| R-free | 0.27880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ah2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.377 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.750 | 2.600 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Number of reflections | 3830 | 274 |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.983 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1 M PCTP, 25 % w/v PEG 1500 |
