7AHS
titin-N2A Ig81-Ig83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-03-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.203, 87.336, 161.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.530 - 2.050 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5joe |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | XSCALE |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.670 | 2.123 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 747399 | 72724 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.8 | |
| CC(1/2) | 0.998 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 25 % w/v Polyethylene glycol 3,350, 100 mM BIS-TRIS pH 5.5, 200 mM lithium sulfate |
