7AHK
Crystal structure of the outward-facing state of the substrate-free Na+-only bound glutamate transporter homolog GltPh
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 152.011, 152.011, 57.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nww |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.870 | 45.870 | 2.600 |
| High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
| Rmerge | 0.171 | 0.021 | 1.365 |
| Rmeas | 0.211 | 0.026 | 1.684 |
| Rpim | 0.122 | 0.015 | 0.968 |
| Total number of observations | 65714 | 1352 | 7580 |
| Number of reflections | 25571 | 537 | 2905 |
| <I/σ(I)> | 5.4 | 26.4 | 0.8 |
| Completeness [%] | 96.4 | 88.4 | 98.5 |
| Redundancy | 2.6 | 2.5 | 2.6 |
| CC(1/2) | 0.990 | 0.999 | 0.250 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.2 | 293 | 1M KH2PO4/Na2HPO4, pH 5.2 |
