7AH8
NF-Y bound to suramin inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.983998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.697, 61.213, 123.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.478 - 2.700 |
| R-factor | 0.225093115344 |
| Rwork | 0.223 |
| R-free | 0.27439 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n1j |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS (0.68) |
| Data scaling software | Aimless (1.12.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.750 |
| Number of reflections | 10061 | 1299 |
| <I/σ(I)> | 16.2 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 200 mM Ammonium citrate pH 7.0, 20% PEG 3350 |
