7AGY

Structure of the N-domain of the K+/H+ antiporter subunit KhtT at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978566
Spacegroup name	P 21 21 21
Unit cell lengths	35.197, 63.594, 64.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.284 - 1.540
R-factor	0.2252
Rwork	0.225
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7agv
RMSD bond length	0.006
RMSD bond angle	0.983
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.310	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.051	1.048
Rmeas	0.053	1.089
Rpim	0.015	0.295
Number of reflections	22120	1075
<I/σ(I)>	20.7	1.6
Completeness [%]	99.8
Redundancy	12.9
CC(1/2)	0.999	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-Tris propane pH 8.5 200 mM KNa tartrate 20% PEG 3350