7AG9

Structure of the Kar9 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	125.621, 125.621, 165.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.860 - 3.300
R-factor	0.2659
Rwork	0.264
R-free	0.28900
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	3.400
High resolution limit [Å]	3.300	3.300
Number of reflections	38506	12118
<I/σ(I)>	11.09
Completeness [%]	99.8
Redundancy	12.3
CC(1/2)	0.999	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 mM Tris-HCl, pH 7.5, 500 mM NaCl supplemented with 250 mM gamma amino butyric acid (GABA).