7ADN

apo tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97950
Spacegroup name	P 65 2 2
Unit cell lengths	127.864, 127.864, 115.486
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.850 - 1.920
R-factor	0.1724
Rwork	0.172
R-free	0.18760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pud
RMSD bond length	0.007
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.850	2.030
High resolution limit [Å]	1.920	1.920
Number of reflections	43128	6777
<I/σ(I)>	31.6
Completeness [%]	99.7	98.5
Redundancy	24.8	20
CC(1/2)	0.999	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283.15	0.1 M magnesium formate