7AC5

Structure of Tubulin Darpin complex 1 collected by rotation serial crystallography on a COC membrane at a synchrotron source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	73.320, 92.030, 83.540
Unit cell angles	90.00, 96.78, 90.00

Refinement procedure

Resolution	49.212 - 2.260
R-factor	0.2179
Rwork	0.216
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nqu
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.212	2.320
High resolution limit [Å]	2.260	2.260
Number of reflections	49614	4049
<I/σ(I)>	4.48	0.64
Completeness [%]	94.1
Redundancy	8.22
CC(1/2)	0.974	0.126

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	26% PEG 3000, 0.2 M ammonium sulfate and 0.1 M bis-tris methane pH 5.5