7ABT
Structure of PPIA in complex with PR dipeptide repeat
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 83.319, 83.319, 52.455 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.465 - 1.310 |
R-factor | 0.135 |
Rwork | 0.134 |
R-free | 0.16550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kul |
RMSD bond length | 0.018 |
RMSD bond angle | 2.137 |
Data reduction software | XDS |
Data scaling software | XPREP |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.465 | 42.430 | 1.330 |
High resolution limit [Å] | 1.310 | 3.610 | 1.310 |
Rmerge | 0.063 | 0.042 | 0.868 |
Number of reflections | 49424 | 2497 | 1177 |
<I/σ(I)> | 21.55 | 73.14 | 1.16 |
Completeness [%] | 98.7 | 99.9 | 91 |
Redundancy | 20.06 | 19.88 | 17.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 25% PEG1000 |